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mouse anti gapdh  (Proteintech)


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    Structured Review

    Proteintech mouse anti gapdh
    Mouse Anti Gapdh, supplied by Proteintech, used in various techniques. Bioz Stars score: 98/100, based on 10577 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gapdh+mouse+monoclonal+antibody/GAPDH+Monoclonal+antibody/pmc13022619-94-0-6
    Average 98 stars, based on 10577 article reviews
    mouse anti gapdh - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Neuron-specific overexpression of human vasoactive intestinal peptide receptor 2 in mice causes cognitive dysfunction and abnormal dendritic morphology in the prefrontal cortex.
    Article Snippet: .. The blotted membranes were blocked with a blocking buffer (Blocking One, #0395395; Nacalai Tesque, Inc) for 30 min at room temperature and subsequently incubated at 4 ◦C overnight with primary antibodies: VPAC2 rabbit monoclonal antibody (1:1000, #ab183334; Abcam) or GAPDH mouse monoclonal antibody (1:20000, #60004-1-Ig; Proteintech, IL, USA). .. The membranes were then incubated with horseradish peroxidase (HRP)conjugated anti-rabbit IgG (1:3000, #7074; Cell Signaling Technology, Beverly, MA, USA) or HRP-conjugated anti-mouse IgG (1:2000, #7076; Cell Signaling Technology) for 1 h at room temperature.

    Incubation:

    Article Title: Neuron-specific overexpression of human vasoactive intestinal peptide receptor 2 in mice causes cognitive dysfunction and abnormal dendritic morphology in the prefrontal cortex.
    Article Snippet: .. The blotted membranes were blocked with a blocking buffer (Blocking One, #0395395; Nacalai Tesque, Inc) for 30 min at room temperature and subsequently incubated at 4 ◦C overnight with primary antibodies: VPAC2 rabbit monoclonal antibody (1:1000, #ab183334; Abcam) or GAPDH mouse monoclonal antibody (1:20000, #60004-1-Ig; Proteintech, IL, USA). .. The membranes were then incubated with horseradish peroxidase (HRP)conjugated anti-rabbit IgG (1:3000, #7074; Cell Signaling Technology, Beverly, MA, USA) or HRP-conjugated anti-mouse IgG (1:2000, #7076; Cell Signaling Technology) for 1 h at room temperature.

    Western Blot:

    Article Title: Transplanting ANXA1⁻ CD8⁺ Naïve T cells Delay Aging Through Senolysis
    Article Snippet: .. For immunoblotting, cultured cells were lysed in Cell lysis buffer for Western and IP (Beyotime cat. P0013) containing a protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific cat. 78440). anti-CDKN2A/p16 INK4a antibody (abcam, cat. ab189034, 1:1000 dilution), GAPDH Mouse Monoclonal Antibody (Proteintech, cat. 60004-1-Ig-100ul, 1:10000 dilution), Alpha Tubulin Monoclonal antibody (Proteintech, cat. 66031-1-Ig, 1:1000 dilution), and HRP-labeled goat anti-mouse IgG (H + L) (Beyotime cat. A0216, 1:2000 dilution) were used as antibodies. .. The signal was detected with ECL Western Blotting Substrate (Thermo Fisher Scientific cat. 34095) and an Amersham Imager 600 (GE Healthcare).

    Cell Culture:

    Article Title: Transplanting ANXA1⁻ CD8⁺ Naïve T cells Delay Aging Through Senolysis
    Article Snippet: .. For immunoblotting, cultured cells were lysed in Cell lysis buffer for Western and IP (Beyotime cat. P0013) containing a protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific cat. 78440). anti-CDKN2A/p16 INK4a antibody (abcam, cat. ab189034, 1:1000 dilution), GAPDH Mouse Monoclonal Antibody (Proteintech, cat. 60004-1-Ig-100ul, 1:10000 dilution), Alpha Tubulin Monoclonal antibody (Proteintech, cat. 66031-1-Ig, 1:1000 dilution), and HRP-labeled goat anti-mouse IgG (H + L) (Beyotime cat. A0216, 1:2000 dilution) were used as antibodies. .. The signal was detected with ECL Western Blotting Substrate (Thermo Fisher Scientific cat. 34095) and an Amersham Imager 600 (GE Healthcare).

    Lysis:

    Article Title: Transplanting ANXA1⁻ CD8⁺ Naïve T cells Delay Aging Through Senolysis
    Article Snippet: .. For immunoblotting, cultured cells were lysed in Cell lysis buffer for Western and IP (Beyotime cat. P0013) containing a protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific cat. 78440). anti-CDKN2A/p16 INK4a antibody (abcam, cat. ab189034, 1:1000 dilution), GAPDH Mouse Monoclonal Antibody (Proteintech, cat. 60004-1-Ig-100ul, 1:10000 dilution), Alpha Tubulin Monoclonal antibody (Proteintech, cat. 66031-1-Ig, 1:1000 dilution), and HRP-labeled goat anti-mouse IgG (H + L) (Beyotime cat. A0216, 1:2000 dilution) were used as antibodies. .. The signal was detected with ECL Western Blotting Substrate (Thermo Fisher Scientific cat. 34095) and an Amersham Imager 600 (GE Healthcare).



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